The complete genome sequence of Listeria monocytogenes strain S2542 and expression of selected genes under high-pressure processing

Table 1 Comparison of two studies [8, 9] and ddPCR-based expression changes for strain RO15, ScottA and S2542

(a) log₂ fold change of the indicated gene after HPP at 400 MPa as determined by RNA-seq for RO15 and ScottA (Duru et al. [8]) or microarray for S2542 (Bowman et al. [9])
Gene	RO15 (RNA-seq, Duru et al. [8]) log₂ fold change	ScottA (RNA-seq, Duru et al. [8]) log₂ fold change	S2542 (microarray, Bowman et al. [9]) log₂ fold change
agrB	− 0.35	− 0.15	2.84
hly	0.89	2.57	− 4.34
ftsE	− 1.11	− 0.66	2.71
clpE	2.26	2.27	− 3.93
mscL	0.24	0.33	1.04
Pearson correlation with S2542	− 0.87	− 0.99

(b) log₂ fold change of the indicated gene immediately after HPP at 400 MPa as determined by ddPCR for all three strains (this study)
Gene	RO15	ScottA	S2542
agrB	− 0.10	− 0.16	− 0.32
hly	− 0.02	0.34	0.36
ftsE	− 0.30	− 0.06	0.20
clpE	0.27	0.27	0.57
mscL	0.01	− 0.73	0.83
Pearson correlation with S2542	0.49	− 0.24

(c) log₂ fold change of the indicated gene immediately after HPP at 400 MPa and storage for 24 h as determined by ddPCR for all three strains (this study)
Gene	RO15	ScottA	S2542
agrB	0.36	0.09	0.59
hly	− 0.06	0.08	0.45
ftsE	0.02	0.60	1.78
clpE	0.28	0.21	0.35
mscL	0.57	0.82	2.30
Pearson correlation with S2542	0.38	0.97

a log₂ fold change results of the two different studies [8, 9] with samples treated at 400 MPa and correlation of the RNAseq results to the DNA microarray results. b ddPCR log₂ fold change results at 0 min after 400 MPa treatment comparison with data from strains RO15, ScottA and S2542 showing the correlation of the gene expression between them. c ddPCR log₂ fold change results at 24 h after 400 MPa treatment with data from strains RO15, ScottA, and S2542 showing the correlation of the gene expression between them

ISSN: 1756-0500